yusZ [2019-04-16 13:35:07]

putative short-chain acyl dehydrogenase

locus: BSU32980

pI: 9.17

mw: 30.64 kDa

protein length: 280 aa

gene length: 843 bp

function: unknown

product: unknown

essential: no

synonyms: yvxA

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,382,633 → 3,383,475

The protein

Protein family

• short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|B7B1D28E50244704324920E5A6F110DA8D63F6C0|YxjF]

[SW|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|3M1A] (from ''Streptomyces avemitilis'', 35% identity)

Expression and Regulation

Operons

• genes: [gene|D945CAD7209CB94A7B9E23263476124B7E8853DD|yusZ]-[gene|68EFECAC6B771D7BDCCD7FD30511152EDEFCEE5B|mrgA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B210 (yusZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1209 NBRP B. subtilis, Japan]
• BKE32980 (Δ[gene|D945CAD7209CB94A7B9E23263476124B7E8853DD|yusZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTAACCCTCCTATTAA, downstream forward: _UP4_TGATCTAAATTATAATTATT
• BKK32980 (Δ[gene|D945CAD7209CB94A7B9E23263476124B7E8853DD|yusZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTAACCCTCCTATTAA, downstream forward: _UP4_TGATCTAAATTATAATTATT